ADA2 isoform of adenosine deaminase from pleural fluid.
FEBS Lett
; 579(3): 643-7, 2005 Jan 31.
Article
em En
| MEDLINE
| ID: mdl-15670822
ABSTRACT
Adenosine deaminase isoenzyme 2 (ADA2) was isolated from human pleural fluid for the first time. Molecular and kinetic properties were characterized. It was shown that the inhibitors of adenosine deaminase isoenzyme 1 (ADA1), adenosine, and erithro-9-(2-hydroxy-3-nonyl)adenine (EHNA) derivatives are poor inhibitors of ADA2. Comparison of the interaction of ADA2 and ADA1 with adenosine and its derivative, 1-deazaadenosine, indicates that the isoenzymes have similar active centers. The absence of ADA2 inhibition by EHNA is evidence of a difference of these active centers in a close environment. The possible role of Zn2+ ions and the participation of acidic amino acids Glu and Asp in adenosine deamination catalyzed by ADA2 were shown.
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Base de dados:
MEDLINE
Assunto principal:
Pleura
/
Adenosina Desaminase
/
Isoenzimas
Limite:
Humans
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article