Cell-type specific expression of IGF-1R in porcine islet cells.
Growth Horm IGF Res
; 15(1): 33-8, 2005 Feb.
Article
em En
| MEDLINE
| ID: mdl-15701570
ABSTRACT
Insulin-like growth factor-1 (IGF-1) functions as a growth factor regarding physiological regulations of cellular metabolism, regeneration and growth. In pancreas islets their potential function is unclear and only little information is available on occurrence and distribution of the corresponding insulin-like growth factor-1 receptor (IGF-1R) in islet cells. Therefore, we investigated the localization of IGF-1R by immunohistochemical techniques and its possible co-localization with other islet hormones. Further, we applied molecular biology techniques to determine the present of local gene expression of IGF-1R and IGF-1. Immunostaining on serial sections with anti-insulin, anti-glucagon and anti-somatostatin antibodies shows, IGF-1R was selectively expressed in insulin-producing B-cells and additionally more pronounced in somatostatin-containing D-cells, which are located in the periphery of porcine pancreatic islets. Furthermore, the RT-PCR experiment demonstrates clearly that IGF-1 and IGF-1R was expressed together in the porcine pancreas. The high expression of IGF-1R in porcine D-cells indicates that mammalian IGF-1R genes are regulated in a different manner since it was shown that in all other species IGF-1R was expressed in B- and A-cells but not in D-cells.
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Base de dados:
MEDLINE
Assunto principal:
Regulação da Expressão Gênica
/
Ilhotas Pancreáticas
/
Receptor IGF Tipo 1
Limite:
Animals
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article