Association between sequence variations in genes encoding human zona pellucida glycoproteins and fertilization failure in IVF.
Hum Reprod
; 20(6): 1578-85, 2005 Jun.
Article
em En
| MEDLINE
| ID: mdl-15860499
ABSTRACT
BACKGROUND:
The zona pellucida (ZP) has multiple roles in reproductive processes, including oocyte maturation, fertilization and implantation. We used, for the first time, a genetic approach to study whether human ZP genes possess structural alterations in women with unsuccessful IVF trials. In theory, this may result in gradual reduction of sperm-zona interaction and eventually in total fertilization failure (TFF).METHODS:
Eighteen infertile women (TFFs) whose IVF did not result in any fertilized oocytes, whereas fertilization by ICSI was successful, were screened for mutations in ZP genes by means of conformation-sensitive gel electrophoresis. Twenty-three fertilizers in IVF (FIVFs) and 68 women with proven fertility (WPFs) constituted the two control groups.RESULTS:
Altogether, 20 sequence variations were found in the ZP genes. Two variations in ZP3, one in the regulatory region (c. 1-87 T --> G) and one in exon 6 [c. 894 G --> A (p. K298)] existed more frequently in TFFs than in FIVF and WPF groups (P-values 0.027 and 0.008, respectively).CONCLUSIONS:
Our study on ZP genes of infertile women revealed a high degree of sequence variations. This may reflect gradual reduction of fertility among TFFs, but the putative roles and influences of single variations can only be hypothesized.
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Base de dados:
MEDLINE
Assunto principal:
Variação Genética
/
Glicoproteínas de Membrana
/
Proteínas do Ovo
/
Fertilização in vitro
/
Receptores de Superfície Celular
/
Infertilidade Feminina
Tipo de estudo:
Observational_studies
/
Risk_factors_studies
Limite:
Adult
/
Female
/
Humans
/
Pregnancy
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article