A natural mutant of plasmid RP4 that confers phage resistance and reduced conjugative transfer.
FEMS Microbiol Lett
; 70(2): 97-100, 1992 Mar 01.
Article
em En
| MEDLINE
| ID: mdl-1587464
ABSTRACT
A natural isolate of RP4 (PRC#116) acquired from the Stanford University Plasmid Reference Center differed from the wild-type Incompatibility Group P plasmid in several respects. Cells of Escherichia coli harboring PRC#116 were resistant to the IncP pili-specific bacteriophage PRD1 and GU5, and transferred this plasmid at a lower efficiency than the wild-type RP4. Phage sensitivity was restored, and transfer considerably improved in PRC#116+ bacteria transformed with plasmid constructs containing the origin of transfer (oriT region) of RP4. Mutant RP4 plasmids equivalent to PRC#116 were selected at a high frequency from an RP4+ E. coli population infected with PRD1 indicating that this RP4 variant may be the product of a very common mutation of the wild-type plasmid.
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Base de dados:
MEDLINE
Assunto principal:
Fatores R
/
Conjugação Genética
/
Escherichia coli
/
Mutação
Idioma:
En
Ano de publicação:
1992
Tipo de documento:
Article