Inhibition by N'-nitrosonornicotine of the catalytic activity of glutamate dehydrogenase in alpha-ketoglutarate amination.
J Enzyme Inhib Med Chem
; 20(1): 89-94, 2005 Feb.
Article
em En
| MEDLINE
| ID: mdl-15895690
ABSTRACT
The effect of N'-nitrosonornicotine (NNN), one of the tobacco-specific nitrosamines, on the catalytic activity of glutamate dehydrogenase (GLDH) in the alpha-ketoglutarate amination, using reduced nicotinamide adenine dinucleotide as coenzyme, was studied by a chronoamperometric method. The maximum reaction rate of the enzyme-catalyzed reaction and the Michaelis-Menten constant, or the apparent Michaelis-Menten constant, were determined in the absence and presence of NNN. NNN remarkably inhibited the bio-catalysis activity of GLDH, and was a reversible competitive inhibitior with K(i), estimated as 199 micromol l(-1) at 25 degrees C and pH 8.0.
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Base de dados:
MEDLINE
Assunto principal:
Inibidores Enzimáticos
/
Glutamato Desidrogenase
/
Nitrosaminas
Limite:
Animals
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article