The stabilization of housekeeping transcripts in Trypanosoma cruzi epimastigotes evidences a global regulation of RNA decay during stationary phase.
FEMS Microbiol Lett
; 246(2): 259-64, 2005 May 15.
Article
em En
| MEDLINE
| ID: mdl-15899414
ABSTRACT
The relative steady state concentration of mRNAs of four housekeeping single-copy type Trypanosoma cruzi genes (actin, triosephosphate isomerase, trypanothion reductase and the ribosomal protein S4) was analyzed throughout the growth curve. A distinguishable pattern was observed with maximal levels occurring at the logarithmic phase of growth and minimum levels occurring at the stationary phase. The half-lives of all analyzed messenger RNAs, and also of three molecular species of immature ribosomal RNAs were increased in cells isolated from stationary phase. These results suggest the occurrence of a novel global regulation mechanism that might protect transcripts from degradation in stationary epimastigotes, probably as a strategy to perpetuate through this quiescent stage.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Trypanosoma cruzi
/
RNA Mensageiro
/
Processamento Pós-Transcricional do RNA
/
Regulação da Expressão Gênica
/
Estabilidade de RNA
Limite:
Animals
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article