The interaction of the hnRNP family member E1B-AP5 with p53.
FEBS Lett
; 579(13): 2752-8, 2005 May 23.
Article
em En
| MEDLINE
| ID: mdl-15907477
ABSTRACT
Adenovirus early region 1B-associated protein 5, E1B-AP5, a member of the heterogeneous nuclear ribonucleoprotein (hnRNP) family, was originally isolated on the basis of its ability to bind to the adenovirus 5 early region1B55K protein. Here, it has been demonstrated that E1B-AP5 interacts with mutant and wild-type p53 from human cells in pull-down assays using GST-E1B-AP5. This interaction has been confirmed by co-immunoprecipitation studies and pull-down experiments with in vitro translated E1B-AP5 and GST-p53. The binding site for E1B-AP5 has been mapped to the C-terminal region of p53. In reciprocal experiments, it has been shown that several regions of E1B-AP5 bound to p53 although it is probable that a major site of interaction is located between amino acids 395 and 732 of E1B-AP5. In reporter assays, E1B-AP5 inhibited p53 transcriptional activity although not as efficiently as the Ad5E1B55K protein. Transfection of E1B-AP5 into human tumour cells affected the cellular response to UV radiation, such that, although p53 expression was induced, little change in the level of p53-inducible genes could be observed.
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Base de dados:
MEDLINE
Assunto principal:
Proteína Supressora de Tumor p53
/
Ribonucleoproteínas Nucleares Heterogêneas
Limite:
Humans
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article