G-protein signaling triggered by R5 human immunodeficiency virus type 1 increases virus replication efficiency in primary T lymphocytes.
J Virol
; 79(12): 7938-41, 2005 Jun.
Article
em En
| MEDLINE
| ID: mdl-15919952
ABSTRACT
The binding of R5 envelope to CCR5 during human immunodeficiency virus type 1 (HIV-1) entry provokes cell activation, which has so far been considered to have no effect on virus replication, since signaling-defective CCR5 molecules have been shown to function normally as HIV-1 coreceptors on transformed cells or mitogen-stimulated T lymphocytes. As the background state of activation of these cells might have biased the results, we performed experiments using the same approach but with nonactivated primary T lymphocytes. We now report that the single R126N mutation in the DRY motif, involved in G-protein coupling, results in a signaling-defective CCR5 coreceptor with a drastically impaired capacity to support HIV-1 infection.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Replicação Viral
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Linfócitos T
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Transdução de Sinais
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HIV-1
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Proteínas de Ligação ao GTP
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Receptores CCR5
Limite:
Humans
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article