Encapsulating live cells with water-soluble chitosan in physiological conditions.
J Biotechnol
; 117(4): 355-65, 2005 Jun 29.
Article
em En
| MEDLINE
| ID: mdl-15925718
ABSTRACT
A new class of microcapsules was prepared under physiological conditions by polyelectrolyte complexation between two oppositely-charged, water-soluble polymers. The microcapsules consisted of an inner core of half N-acetylated chitosan and an outer shell of methacrylic acid (MAA) (20.4%)-hydroxyethyl methacrylate (HEMA) (27.4%)-methyl methacrylate (MMA) (52.2%) (MAA-HEMA-MMA) terpolymer. Both 400 and 150 kDa half N-acetylated chitosans maintained good water solubility and supplied enough protonated amino groups to coacervate with terpolymer at pH 7.0-7.4, in contrast to other chitosan-based microcapsules which must be prepared at pH <6.5. The viscosity of half N-acetylated chitosan solutions between 80 and 3000 cPas allowed the formation of microcapsules with spherical shape. Molar mass, pH and concentration of half N-acetylated chitosan, and reaction time, influenced the morphology, thickness and porosity of the microcapsules. Microcapsules formed with high concentration of half N-acetylated chitosan exhibited improved mechanical stability, whereas microcapsules formed with low concentration of half N-acetylated chitosan exhibited good permeability. This 3D microenvironment has been configured to cultivate sensitive anchorage-dependent cells such as hepatocytes to maintain high level of functions.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Ácidos Polimetacrílicos
/
Materiais Biocompatíveis
/
Água
/
Técnicas de Cultura de Células
/
Hepatócitos
/
Engenharia Tecidual
/
Quitosana
Limite:
Animals
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article