The development of functional B lymphocytes in conditional PU.1 knock-out mice.
Blood
; 106(6): 2083-90, 2005 Sep 15.
Article
em En
| MEDLINE
| ID: mdl-15933053
ABSTRACT
An abundance of research has entrenched the view that the Ets domain containing transcription factor PU.1 is fundamental to the development and function of B lymphocytes. In this study, we have made use of a conditional PU.1 allele to test this notion. Complete deletion of PU.1 resulted in the loss of B cells and all other lineage-positive cells in the fetal liver and death between E18.5 and birth; however, specific deletion of PU.1 in the B lineage had no effect on B-cell development. Furthermore, deletion of PU.1 in B cells did not compromise their ability to establish and maintain an immune response. An increased level of apoptosis was observed in vitro upon B-cell receptor (BCR) cross-linking; however, this was partially rescued by interleukin-4 (IL-4). These findings suggest that PU.1 is not essential for the development of functional B lymphocytes beyond the pre-B stage.
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Base de dados:
MEDLINE
Assunto principal:
Linfócitos B
/
Transativadores
/
Proteínas Proto-Oncogênicas
Limite:
Animals
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article