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Chemokine-induced recruitment of genetically modified bone marrow cells into the CNS of GM1-gangliosidosis mice corrects neuronal pathology.
Sano, Renata; Tessitore, Alessandra; Ingrassia, Angela; d'Azzo, Alessandra.
Afiliação
  • Sano R; Department of Genetics and Tumor Cell Biology, St Jude Children's Research Hospital, Memphis, TN 38105, USA.
Blood ; 106(7): 2259-68, 2005 Oct 01.
Article em En | MEDLINE | ID: mdl-15941905
Bone marrow cells (BMCs) could correct some pathologic conditions of the central nervous system (CNS) if these cells would effectively repopulate the brain. One such condition is G(M1)-gangliosidosis, a neurodegenerative glycosphingolipidosis due to deficiency of lysosomal beta-galactosidase (beta-gal). In this disease, abnormal build up of G(M1)-ganglioside in the endoplasmic reticulum of brain cells results in calcium imbalance, induction of an unfolded protein response (UPR), and neuronal apoptosis. These processes are accompanied by the activation/proliferation of microglia and the production of inflammatory cytokines. Here we demonstrate that local neuroinflammation promotes the selective activation of chemokines, such as stromal-cell-derived factor 1 (SDF-1), macrophage inflammatory protein 1-alpha (MIP-1alpha), and MIP-1beta, which chemoattract genetically modified BMCs into the CNS. Mice that underwent bone marrow transplantation showed increased beta-gal activity in different brain regions and reduced lysosomal storage. Decreased production of chemokines and effectors of the UPR as well as restoration of neurologic functions accompanied this phenotypic reversion. Our results suggest that beta-gal-expressing bone marrow (BM)-derived cells selectively migrate to the CNS under a gradient of chemokines and become a source of correcting enzyme to deficient neurons. Thus, a disease condition such as G(M1)-gangliosidosis, which is characterized by neurodegeneration and neuroinflammation, may influence the response of the CNS to ex vivo gene therapy.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células da Medula Óssea / Sistema Nervoso Central / Gangliosidose GM1 / Quimiocinas Idioma: En Ano de publicação: 2005 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Células da Medula Óssea / Sistema Nervoso Central / Gangliosidose GM1 / Quimiocinas Idioma: En Ano de publicação: 2005 Tipo de documento: Article