Doxycycline, the drug used to control the tet-regulatable promoter system, has no effect on global gene expression in Saccharomyces cerevisiae.
Yeast
; 22(7): 565-9, 2005 May.
Article
em En
| MEDLINE
| ID: mdl-15942933
ABSTRACT
The tet-regulatable promoter system is commonly used for genetic studies in many eukaryotic organisms. The promoter is regulated using doxycycline. There are no obvious phenotypic effects observed when doxycycline is added to the growth medium of yeast to control expression from the promoter. It is widely accepted that doxycycline is innocuous to yeast. Global genetic studies are now commonplace and the tetO-system is being used in transcriptome studies. Hence, we wanted to ensure that the absence of phenotypic effects, on addition of doxycycline to the growth medium, is mirrored in transcriptome data. We have demonstrated that doxycycline has no significant effect on global transcription levels and will continue to use the tetO-regulatable promoter system for genetic studies.
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Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
/
Regulação Fúngica da Expressão Gênica
/
Doxiciclina
/
Proteoma
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Proteínas de Saccharomyces cerevisiae
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article