Growth regulation of human renal carcinoma cells: role of transforming growth factor alpha.
Cancer Res
; 52(12): 3335-9, 1992 Jun 15.
Article
em En
| MEDLINE
| ID: mdl-1596891
Findings of increased numbers of epidermal growth factor receptors (EGF-R) and increased expression of transforming growth factor alpha (TGF-alpha) in surgical specimens of human renal cell carcinoma have led to the proposal that growth of these tumors may be regulated by TGF-alpha in an autocrine manner. In the studies presented here, we have examined this hypothesis using two human renal carcinoma cell lines, SKRC-4 and SKRC-29. We demonstrated that both SKRC-4 and SKRC-29 cells were growth stimulated by greater than 35% when cultured in the presence of TGF-alpha or EGF and were inhibited by 29% to 46% if cultured in the presence of anti-EGF-R monoclonal antibody 225. Treatment of cells with TGF-alpha enhanced the levels of expression of EGF-R mRNA and TGF-alpha mRNA. In addition, incubation of cells with monoclonal antibody 225 significantly elevated the levels of excreted TGF-alpha species in the culture medium. Our findings suggest that proliferation of human renal carcinoma cells may be regulated by endogenously produced TGF-alpha and that this regulatory pathway can be interrupted using antibody to its receptor, EGF-R.
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Base de dados:
MEDLINE
Assunto principal:
RNA Mensageiro
/
Carcinoma de Células Renais
/
Fator de Crescimento Transformador alfa
/
Receptores ErbB
Limite:
Humans
Idioma:
En
Ano de publicação:
1992
Tipo de documento:
Article