Isolation and structure determination of bioactive isoflavones from callus culture of Dipteryx odorata.
Chem Pharm Bull (Tokyo)
; 53(7): 740-2, 2005 Jul.
Article
em En
| MEDLINE
| ID: mdl-15997126
ABSTRACT
Crude extracts of callus and roots of Dipteryx odorata were analyzed by HPLC to detect and quantify isoflavone contents. Based on spectroscopic and X-ray crystallography data the structures of two isoflavones were elucidated as 7-hydroxy-4',6-dimethoxyisoflavone and 3',7-dihydroxy-4',6-dimethoxyisoflavone. The production of dry biomass of 7-hydroxy-4',6-dimethoxyisoflavone in cultured callus was 4.12 mg/g, approximately eleven fold higher than the amount accumulated in roots of D. odorata wild-growing plants. The 7-hydroxy-4',6-dimethoxyisoflavone was effective against glyceraldehyde-3-phosphate dehydrogenase from Trypanosoma cruzi. This is the first report on those bioactive isoflavones accumulated in callus of D. odorata.
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Base de dados:
MEDLINE
Assunto principal:
Dipteryx
/
Isoflavonas
Limite:
Animals
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article