[Expression of C II TA gene in five human malignant hematological cell lines and its significance].
Zhejiang Da Xue Xue Bao Yi Xue Ban
; 34(4): 344-7, 2005 07.
Article
em Zh
| MEDLINE
| ID: mdl-16059983
ABSTRACT
OBJECTIVE:
To investigate the role of MHC class II Transactivator (C II TA) in expression of HLA molecules in five human malignant hematological cell lines.METHODS:
The expressions of HLA molecules and C II TA protein were detected by immunohistochemistry and flow cytometry. The expression of C II TA gene was detected by RT-PCR. The response of peripheral T cells after stimulation by Jurkat cells was detected by mixed lymphocyte reaction.RESULT:
The HLA II-positive tumor cells expressed the C II TA and IFN-gamma induced the expression of HLA I, II in tumor cells, which were able to express C II TA constitutively.CONCLUSION:
There is a correlation between the inability of the tumor cells in response to IFN-gamma for HLA expression and the deficiency in the inducible expression of C II TA.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Nucleares
/
Leucemia
/
Transativadores
/
Genes MHC da Classe II
Limite:
Humans
Idioma:
Zh
Ano de publicação:
2005
Tipo de documento:
Article