Rosmarinic acid inhibits the formation of reactive oxygen and nitrogen species in RAW264.7 macrophages.
Free Radic Res
; 39(9): 995-1003, 2005 Sep.
Article
em En
| MEDLINE
| ID: mdl-16087481
ABSTRACT
Antioxidant action of Rosmarinic acid (Ros A), a natural phenolic ingredient in many Lamiaceae herbs such as Perilla frutescens, sage, basil and mint, was analyzed in relation to the Ikappa-B activation in RAW264.7 macrophages. Ros A inhibited nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) protein synthesis induced by lipopolysaccharide (LPS), and also effectively suppressed phorbol 12-myristate 13-acetate (PMA)-induced superoxide production in RAW264.7 macrophages in a dose-dependent manner. Peroxynitrite-induced formation of 3-nitrotyrosine in bovine serum albumin and RAW264.7 macrophages were also inhibited by Ros A. Moreover, Western blot analysis demonstrated that LPS-induced phosphorylation of Ikappa-Balpha was abolished by Ros A. Ros A can act as an effective protector against peroxynitrite-mediated damage, and as a potent inhibitor of superoxide and NO synthesis; the inhibition of the formation of reactive oxygen and nitrogen species are partly based on its ability to inhibit the serine phosphorylation of Ikappa-Balpha.
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Base de dados:
MEDLINE
Assunto principal:
Cinamatos
/
Espécies Reativas de Oxigênio
/
Espécies Reativas de Nitrogênio
/
Macrófagos
/
Antioxidantes
Limite:
Animals
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article