[Isolation and characterization of new species hydrogen producing bacterium Ethanologenbacterium sp. strain X-1 and its capability of hydrogen production].
Wei Sheng Wu Xue Bao
; 44(6): 724-8, 2004 Dec.
Article
em Zh
| MEDLINE
| ID: mdl-16110947
ABSTRACT
To obtain hydrogen-producing bacterium of high efficiency, a strain X-1 of hydrogen-producing bacteria was isolated from the continuous stirred-tank reactor (CSTR) by anaerobic Hungate technique. The Comparative sequence analysis of 16S rDNA showed that homology of strain X-1 with Clostridium cellulose and Acetanaerobacterium elongatum is less than 94%. All sequence alignment of 16S-23S rDNA intergenic spacer regions (ISR) indicated displayed that consensus region is tRNA(Ala), and tRNA(Ile), variable region is not homologous. Morphological, physic-biochemical character, and comparative sequence analysis of 16S rDNA and 16S-23S rDNA ISR indicated that strain X-1 belong to new genus named Ethanologenbacterium gen. nov.. Strain X-1 is facultative anaerobe bacillus; its main fermentative products are acetic acid, ethanol, H2 and CO2. The metabolic character of strain X-1 is typical ethanol type fermentation. Its capability of hydrogen production was measured in the batch culture experiment. X-1's maximum specific hydrogen producing rate is 28.3 mmol H2/( g dry cell x h) at pH 4.0 and 36 degrees C. Result of identify and analysis of hydrogen production ability demonstrated strain X-1 belong to new genus of high hydrogen-producing bacteria.
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Base de dados:
MEDLINE
Assunto principal:
Etanol
/
Fermentação
/
Bactérias Gram-Positivas
Tipo de estudo:
Prognostic_studies
Idioma:
Zh
Ano de publicação:
2004
Tipo de documento:
Article