Detection of Brazilian spotted fever infection by polymerase chain reaction in a patient from the state of São Paulo.
Mem Inst Oswaldo Cruz
; 100(3): 277-9, 2005 May.
Article
em En
| MEDLINE
| ID: mdl-16113868
ABSTRACT
Brazilian spotted fever (BSF) cases have been increasing in the state of São Paulo but no genomic information about local rickettsia isolated from humans has been well documented. We recovered spotted-fever group rickettsiae from a sample of patient blood cultured in Vero cells using the shell vial technique. Rickettsial DNA fragments (gltA, ompA, and, ompB genes) were detected, and analysis of the ompB gene base sequences showed identity with the Rickettsia rickettsii ompB sequence available in the GenBank.
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Base de dados:
MEDLINE
Assunto principal:
Rickettsia rickettsii
/
Infecções por Rickettsia
/
Proteínas da Membrana Bacteriana Externa
/
DNA Bacteriano
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
/
Child, preschool
/
Humans
/
Male
País como assunto:
America do sul
/
Brasil
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article