[Analysis of succeed percentage on sequencing ESTs and construction of porcine muscle cDNA library].
Yi Chuan
; 24(3): 263-6, 2002 May.
Article
em Zh
| MEDLINE
| ID: mdl-16126678
ABSTRACT
Using Longissimus Dorsi muscle as material and Lambda ZAP II as Vector, Xiang Pig Longissimus Dorsi muscle cDNA library has been constructed in our study. The results showed that the titration of the library was 3.4 x 10(7) pfu/ml, the recombinant percentage was 94%, and the fragment length of inserted average cDNA were 1.5 kb. The study pointed out that the more than 30 T insertion is the major factor for low percentage if sequencing the 3'-end.
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Base de dados:
MEDLINE
Idioma:
Zh
Ano de publicação:
2002
Tipo de documento:
Article