Apoptosis in Vero cells infected with Akabane, Aino and Chuzan virus.
J Vet Sci
; 6(3): 251-4, 2005 Sep.
Article
em En
| MEDLINE
| ID: mdl-16131831
ABSTRACT
Akabane, Aino and Chuzan virus are arthropod-borne (arbo) viruses mainly associated with reproductive failures in cattle. We investigated apoptosis in Vero cells (C-1586) infected with Akabane, Aino and Chuzan virus. The fragmentation of chromosomal DNA was simultaneously detected with the progress of cytopathic effect from 48 hr to 72 hr post infection, depending on viruses. Although the treatment of cycloheximide blocked apoptosis in Vero cells infected with three viruses, actinomycin D did not prevent DNA oligomerization, thus indicating that de novo viral protein synthesis is critical for viral apoptosis. In addition, the activation of caspase-3 was also detected in Vero cells by indirect fluorescent assay. From the present results, it is of future interest whether apoptotic characteristics of these viruses are related to pathogenecity in vivo.
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Base de dados:
MEDLINE
Assunto principal:
Bunyaviridae
/
Apoptose
/
Orbivirus
/
Efeito Citopatogênico Viral
Limite:
Animals
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article