A gravimetric simplified method for nucleated marrow cell counting using an injection needle.
J Toxicol Sci
; 30(3): 261-4, 2005 Aug.
Article
em En
| MEDLINE
| ID: mdl-16141659
ABSTRACT
A simplified gravimetric marrow cell counting method for rats is proposed for a regular screening method. After fresh bone marrow was aspirated by an injection needle, the marrow cells were suspended in carbonate buffered saline. The nucleated marrow cell count (NMC) was measured by an automated multi-blood cell analyzer. When this gravimetric method was applied to rats, the NMC of the left and right femurs had essentially identical values due to careful handling. The NMC at 4 to 10 weeks of age in male and female CrjCD(SD)IGS rats was 2.72 to 1.96 and 2.75 to 1.98 (x10(6) counts/mg), respectively. More useful information for evaluation could be obtained by using this gravimetric method in addition to myelogram examination. However, some difficulties with this method include low NMC due to blood contamination and variation of NMC due to handling. Therefore, the utility of this gravimetric method for screening will be clarified by the accumulation of the data on myelotoxicity studies with this method.
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Base de dados:
MEDLINE
Assunto principal:
Exame de Medula Óssea
/
Células da Medula Óssea
Limite:
Animals
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article