Molecular recognition via triplex formation of mixed purine/pyrimidine DNA sequences using oligoTRIPs.
J Am Chem Soc
; 127(36): 12657-65, 2005 Sep 14.
Article
em En
| MEDLINE
| ID: mdl-16144414
ABSTRACT
Stable DNA triple-helical structures are normally restricted to homopurine sequences. We have described a system of four heterocyclic bases (TRIPsides) that, when incorporated into oligomers (oligoTRIPs), can recognize and bind in the major groove to any native sequence of DNA [Li et al., J. Am. Chem. Soc. 2003, 125, 2084]. To date, we have reported on triplex-forming oligomers composed of two of these TRIPsides, i.e., antiTA and antiGC, and their ability to form intramolecular triplexes at mixed purine/pyrimidine sequences. In the present study, we describe the synthesis and characterization of the antiCG TRIPside and its use in conjunction with antiTA and antiGC to form sequence-specific intra- and/or intermolecular triplex structures at mixed purine/pyrimidine sequences that require as many as four major groove crossovers.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Oligonucleotídeos
/
Purinas
/
Pirimidinas
/
DNA
/
Conformação de Ácido Nucleico
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article