Stable transduction of primary human monocytes by simian lentiviral vector PBj.
Mol Ther
; 12(6): 1206-16, 2005 Dec.
Article
em En
| MEDLINE
| ID: mdl-16150648
ABSTRACT
Despite the ability to infect nonproliferating cells, current lentiviral vectors are inefficient at mediating gene transfer into quiescent primary human cells such as monocytes. Here, a replication-incompetent vector based on a molecular clone of simian immunodeficiency virus strain PBj (SIVsmmPBj1.9) was generated that, in contrast to lenti- and gamma-retroviral control vectors, enabled transfer of heterologous genes into human diploid fibroblasts and cell lines blocked in the G(0) phase of the cell cycle. Moreover, freshly isolated human monocytes refractory to HIV-1-derived vectors were efficiently transduced by the PBj vector independent of the viral Nef protein. Stable chromosomal integration of PBj-derived viral expression vectors was verified in transduced cells. The capability of the PBj vector to transduce quiescent cells such as unstimulated primary human monocytes is an important extension of human gene therapy perspectives.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Monócitos
/
Terapia Genética
/
Lentivirus
/
Vetores Genéticos
Limite:
Humans
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article