Lifespan and stress resistance of Caenorhabditis elegans are increased by expression of glutathione transferases capable of metabolizing the lipid peroxidation product 4-hydroxynonenal.
Aging Cell
; 4(5): 257-71, 2005 Oct.
Article
em En
| MEDLINE
| ID: mdl-16164425
ABSTRACT
Caenorhabditis elegans expresses a glutathione transferase (GST) belonging to the Pi class, for which we propose the name CeGSTP2-2. CeGSTP2-2 (the product of the gst-10 gene) has the ability to conjugate the lipid peroxidation product 4-hydroxynonenal (4-HNE). Transgenic C. elegans strains were generated in which the 5'-flanking region and promoter of gst-10 were placed upstream of gst-10 and mGsta4 cDNAs, respectively. mGsta4 encodes the murine mGSTA4-4, an enzyme with particularly high catalytic efficiency for 4-HNE. The localization of both transgenes was similar to that of native CeGSTP2-2. The 4-HNE-conjugating activity in worm lysates increased in the order control
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Base de dados:
MEDLINE
Assunto principal:
Peroxidação de Lipídeos
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Caenorhabditis elegans
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Proteínas de Caenorhabditis elegans
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Aldeídos
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Glutationa Transferase
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Inibidores do Crescimento
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Longevidade
Limite:
Animals
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article