Nerve growth factor transcriptional control of c-fos promoter transfected in cultured spinal sensory neurons.
J Cell Biol
; 118(1): 131-8, 1992 Jul.
Article
em En
| MEDLINE
| ID: mdl-1618900
ABSTRACT
High efficiency gene transfer (greater than 90%) in chicken dorsal root ganglion neurons has been obtained by DNA calcium phosphate co-precipitation, hence providing an important tool to study control of gene expression in primary neurons. Transfection with c-fos promoter sequences linked to the chloramphenicol acetyltransferase reporter gene showed that the serum responsive element functions as a strong transcriptional enhancer. Transcription from this element is developmentally regulated, and mediates the genetic response to nerve growth factor (NGF) in developing avian sensory neurons. Furthermore, NGF exerts a negative effect on transcription from the cyclic AMP responsive element, thereby supporting the involvement of tyrosine kinase activation by NGF in primary sensory neurons.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Transcrição Gênica
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Transfecção
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Regiões Promotoras Genéticas
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Genes fos
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Fatores de Crescimento Neural
Limite:
Animals
Idioma:
En
Ano de publicação:
1992
Tipo de documento:
Article