Homotypic interaction of Bunyamwera virus nucleocapsid protein.
J Virol
; 79(20): 13166-72, 2005 Oct.
Article
em En
| MEDLINE
| ID: mdl-16189017
ABSTRACT
The bunyavirus nucleocapsid protein, N, plays a central role in viral replication in encapsidating the three genomic RNA segments to form functional templates for transcription and replication by the viral RNA-dependent RNA polymerase. Here we report functional mapping of interacting domains of the Bunyamwera orthobunyavirus N protein by yeast and mammalian two-hybrid systems, immunoprecipitation experiments, and chemical cross-linking studies. N forms a range of multimers from dimers to high-molecular-weight structures, independently of the presence of RNA. Deletion of the N- or C-terminal domains resulted in loss of activity in a minireplicon assay and a decreased capacity for N to form higher multimers. Our data suggest a head-to-head and tail-to-tail multimerization model for the orthobunyavirus N protein.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Vírus Bunyamwera
/
RNA Viral
/
Nucleocapsídeo
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article