Your browser doesn't support javascript.
loading
Kinesin-related proteins required for assembly of the mitotic spindle.
Roof, D M; Meluh, P B; Rose, M D.
Afiliação
  • Roof DM; Department of Molecular Biology, Princeton University, New Jersey 08544.
J Cell Biol ; 118(1): 95-108, 1992 Jul.
Article em En | MEDLINE | ID: mdl-1618910
We identified two new Saccharomyces cerevisiae kinesin-related genes, KIP1 and KIP2, using polymerase chain reaction primers corresponding to highly conserved regions of the kinesin motor domain. Both KIP proteins are expressed in vivo, but deletion mutations conferred no phenotype. Moreover, kip1 kip2 double mutants and a triple mutant with kinesin-related kar3 had no synthetic phenotype. Using a genetic screen for mutations that make KIP1 essential, we identified another gene, KSL2, which proved to be another kinesin-related gene, CIN8. KIP1 and CIN8 are functionally redundant: double mutants arrested in mitosis whereas the single mutants did not. The microtubule organizing centers of arrested cells were duplicated but unseparated, indicating that KIP1 or CIN8 is required for mitotic spindle assembly. Consistent with this role, KIP1 protein was found to colocalize with the mitotic spindle.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Saccharomyces cerevisiae / Família Multigênica / Cinesinas / Proteínas de Saccharomyces cerevisiae / Fuso Acromático Idioma: En Ano de publicação: 1992 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Saccharomyces cerevisiae / Família Multigênica / Cinesinas / Proteínas de Saccharomyces cerevisiae / Fuso Acromático Idioma: En Ano de publicação: 1992 Tipo de documento: Article