Altering the substrate specificity of glutamate dehydrogenase from Bacillus subtilis by site-directed mutagenesis.
Biosci Biotechnol Biochem
; 69(9): 1802-5, 2005 Sep.
Article
em En
| MEDLINE
| ID: mdl-16195607
ABSTRACT
The Lys80, Gly82 and Met101 residues of glutamate dehydrogenase from Bacillus subtilis were mutated into a series of single mutants. The wild-type enzyme was highly specific for 2-oxoglutarate, whereas G82K and M101S dramatically switched to increased specificity for oxaloacetate with kcat values 3.45 and 5.68 s-1, which were 265-fold and 473-fold higher respectively than those for 2-oxoglutarate.
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Base de dados:
MEDLINE
Assunto principal:
Bacillus subtilis
/
Mutagênese Sítio-Dirigida
/
Glutamato Desidrogenase
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article