Cloning of novel bombesin precursor cDNAs from skin of Bombina maxima.
Regul Pept
; 132(1-3): 102-6, 2005 Dec 15.
Article
em En
| MEDLINE
| ID: mdl-16203047
ABSTRACT
Amphibian skin is a rich resource of bioactive peptides like proline-rich bombesin from frog Bombina maxima. A novel cDNA clone encoding a precursor protein that comprises proline-rich bombesin and a novel peptide, designated as bombestatin, was isolated from a skin cDNA library of B. maxima. The predicted primary structure of the novel peptide is WEVLLNVALIRLELLSCRSSKDQDQKESCGMHSW, in which two cysteines form a disulfide bond. A BLAST search of databases did not detect sequences with significant similarity. Bombestatin possesses dose-dependent contractile activity on rat stomach strips. The differences between cDNAs encoding PR-bombesin plus bombestatin and PR-bombesin alone are due to fragment insertions located in 3'-coding region and 3'-untranslational region, respectively.
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Base de dados:
MEDLINE
Assunto principal:
Anuros
/
Pele
/
Bombesina
/
DNA Complementar
/
Proteínas de Anfíbios
Limite:
Animals
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article