Differential immunogenicity of HIV-1 clade C proteins in eliciting CD8+ and CD4+ cell responses.
J Infect Dis
; 192(9): 1588-96, 2005 Nov 01.
Article
em En
| MEDLINE
| ID: mdl-16206073
ABSTRACT
BACKGROUND:
The relative immunogenicity of human immunodeficiency virus type 1 (HIV-1) proteins for CD8+ and CD4+ cell responses has not been defined.METHODS:
HIV-1-specific T cell responses were evaluated in 65 chronically HIV-1-infected untreated subjects by interferon- gamma flow cytometry with peptides spanning the clade C consensus sequence.RESULTS:
The magnitude of HIV-1-specific CD8+ T cell responses correlated significantly with CD4+ cell responses, but the percentage of CD8+ T cells directed against HIV-1 (median, 2.76%) was always greater than that of CD4+ cells (median, 0.24%). Although CD8+ T cell responses were equally distributed among Gag, Pol, and the regulatory and accessory proteins, Gag was the dominant target for CD4+ cell responses. There was no consistent relationship between virus-specific CD8+ or CD4+ cell response and viral load. However, the median viral load in subjects in whom Gag was the dominant CD8+ T cell target was significantly lower than that in subjects in whom non-Gag proteins were the main target (P=.007).CONCLUSIONS:
Gag-specific responses dominate the CD4+ T cell response to HIV, whereas CD8+ T cell responses are broadly distributed, which indicates differential immunogenicity of these cells against HIV-1. The preferential targeting of Gag by CD8+ T cells is associated with enhanced control of viral load.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Virais
/
Linfócitos T CD4-Positivos
/
Infecções por HIV
/
HIV-1
/
Linfócitos T CD8-Positivos
Limite:
Female
/
Humans
/
Male
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article