Evidences for ubiquitination and intracellular trafficking of LAT, the linker of activated T cells.
Biochim Biophys Acta
; 1746(2): 108-15, 2005 Dec 15.
Article
em En
| MEDLINE
| ID: mdl-16236370
ABSTRACT
Current evidences indicate that T cells use protein sorting and degradation to control duration and specificity of T cell receptor (TcR) signalling, including the CD3zeta chain which is ubiquitinated upon TcR triggering. In a previous study, we showed that the Linker of activated T cells (LAT) is present at the plasma membrane and in transferrin-labelled intracellular compartments also containing the CD3zeta chain. Here we show that LAT protein levels are tightly regulated in Jurkat lymphoid T cells likely involving proteasome-dependent degradation, recycling through trans-Golgi/endosome compartments and clathrin-dependent internalisation. We further identify a novel post-translational modification of LAT by ubiquitination that is likely to influence LAT protein stability, intracellular localisation and/or recycling. Our results provide novel molecular and regulatory insights into the function of LAT adapter protein in T cell signalling.
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Base de dados:
MEDLINE
Assunto principal:
Fosfoproteínas
/
Linfócitos T
/
Ubiquitina
/
Proteínas Adaptadoras de Transdução de Sinal
/
Proteínas de Membrana
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article