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Formation of insoluble magnesium phosphates during growth of the archaea Halorubrum distributum and Halobacterium salinarium and the bacterium Brevibacterium antiquum.
Smirnov, Aleksey; Suzina, Natalia; Chudinova, Natalia; Kulakovskaya, Tatiana; Kulaev, Igor.
Afiliação
  • Smirnov A; Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, 142290 Pushchino, Moscow Region, Russia.
FEMS Microbiol Ecol ; 52(1): 129-37, 2005 Mar 01.
Article em En | MEDLINE | ID: mdl-16329899
Stationary phase cells of the halophilic archaea Halobacterium salinarium and Halorubrum distributum, growing at 3-4 M NaCl, and of the halotolerant bacterium Brevibacterium antiquum, growing with and without 2.6 NaCl, took up approximately 90% of the phosphate from the culture media containing 2.3 and 11.5 mM phosphate. The uptake was blocked by the uncoupler FCCP. In B. antiquum, EDTA inhibited the phosphate uptake. The content of polyphosphates in the cells was significantly lower than the content of orthophosphate. At a high phosphate concentration, up to 80% of the phosphate taken up from the culture medium was accumulated as Mg(2)PO(4)OH x 4H(2)O in H. salinarium and H. distributum and as NH(4)MgPO(4) x 6H(2)O in B. antiquum. Consolidation of the cytoplasm and enlargement of the nucleoid zone were observed in the cells during phosphate accumulation. At phosphate surplus, part of the H. salinarium and H. distributum cell population was lysed. The cells of B. antiquum were not lysed and phosphate crystals were observed in the cytoplasm.
Assuntos
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Base de dados: MEDLINE Assunto principal: Fosfatos / Brevibacterium / Archaea / Compostos de Magnésio Idioma: En Ano de publicação: 2005 Tipo de documento: Article
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Base de dados: MEDLINE Assunto principal: Fosfatos / Brevibacterium / Archaea / Compostos de Magnésio Idioma: En Ano de publicação: 2005 Tipo de documento: Article