Cryopreservative effect of leupeptin on early human bone marrow progenitors.
In Vitro Cell Dev Biol
; 28A(6): 459-60, 1992 Jun.
Article
em En
| MEDLINE
| ID: mdl-1634445
ABSTRACT
Leupeptin, a thiol- and serine-proteinase inhibitor of low molecular weight, quickly enters viable cells. This property has been used to protect cells during thawing against intracellular proteolytic activities released by injured lysosomes. The bone marrow nucleated cells were frozen without rate-controlled freezing devices. Concentrations ranging from 0.1 to 1 microM of leupeptin allow to recover 87% of the most immature multipotent bone marrow progenitors which can develop in vitro into large multilineage colonies, instead of 58% recovery without leupeptin. The protective effect of leupeptin is particularly useful to freeze cells difficult to cryopreserve or when freezing-control equipment are not available.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Células-Tronco
/
Células da Medula Óssea
/
Criopreservação
/
Leupeptinas
Limite:
Humans
Idioma:
En
Ano de publicação:
1992
Tipo de documento:
Article