A role for the Rab6A' GTPase in the inactivation of the Mad2-spindle checkpoint.
EMBO J
; 25(2): 278-89, 2006 Jan 25.
Article
em En
| MEDLINE
| ID: mdl-16395330
ABSTRACT
The two isoforms of the Rab6 GTPase, Rab6A and Rab6A', regulate a retrograde transport route connecting early endosomes and the endoplasmic reticulum via the Golgi complex in interphasic cells. Here we report that when Rab6A' function is altered cells are unable to progress normally through mitosis. Such cells are blocked in metaphase, despite displaying a normal Golgi fragmentation and with the Mad2-spindle checkpoint activated. Furthermore, the Rab6 effector p150(Glued), a subunit of the dynein/dynactin complex, remains associated with some kinetochores. A similar phenotype was observed when GAPCenA, a GTPase-activating protein of Rab6, was depleted from cells. Our results suggest that Rab6A' likely regulates the dynamics of the dynein/dynactin complex at the kinetochores and consequently the inactivation of the Mad2-spindle checkpoint. Rab6A', through its interaction with p150(Glued) and GAPCenA, may thus participate in a pathway involved in the metaphase/anaphase transition.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas Repressoras
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Proteínas de Ligação ao Cálcio
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Proteínas de Ciclo Celular
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Proteínas rab de Ligação ao GTP
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Metáfase
Limite:
Humans
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article