Tissue-specific RNAi reveals that WT1 expression in nurse cells controls germ cell survival and spermatogenesis.
Genes Dev
; 20(2): 147-52, 2006 Jan 15.
Article
em En
| MEDLINE
| ID: mdl-16418481
Using a novel tissue-specific RNA interference (RNAi) approach that mimics the principle by which naturally occurring microRNAs (miRNA) are made, we demonstrate that the Wilms' tumor 1 (WT1) transcription factor has an essential role in spermatogenesis. Mice depleted of WT1 in Sertoli nurse cells suffered from increased germ cell apoptosis, loss of adherens junctions, disregulation of adherence junction-associated genes, and impaired fertility. These effects were recapitulated in transgenic mice expressing a dominant-negative form of WT1 in Sertoli cells, demonstrating the validity of our RNAi approach. Our results indicate that the tumor suppressor WT1 promotes Sertoli cell-germ cell signaling events driving spermatogenesis.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Células de Sertoli
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Espermatogênese
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Proteínas WT1
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Interferência de RNA
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Células Germinativas
Limite:
Animals
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article