A checkpoint for autoreactivity in human IgM+ memory B cell development.
J Exp Med
; 203(2): 393-400, 2006 Feb 20.
Article
em En
| MEDLINE
| ID: mdl-16446381
ABSTRACT
Autoantibodies are removed from the repertoire at two checkpoints during B cell development in the bone marrow and the periphery. Despite these checkpoints, up to 20% of the antibodies expressed by mature naive B cells in healthy humans show low levels of self-reactivity. To determine whether self-reactive antibodies are also part of the antigen-experienced memory B cell compartment, we analyzed recombinant antibodies cloned from single circulating human IgM+ memory B cells. Cells expressing antibodies specific for individual bacterial polysaccharides were expanded in the IgM+ memory compartment. In contrast, B cells expressing self-reactive and broadly bacterially reactive antibodies were removed from the repertoire in the transition from naive to IgM+ memory B cell. Selection against self-reactive antibodies was implemented before the onset of somatic hypermutation. We conclude that a third checkpoint selects against self-reactivity during IgM+ memory B cell development in humans.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Autoanticorpos
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Imunoglobulina M
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Linfócitos B
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Diferenciação Celular
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Memória Imunológica
Limite:
Humans
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article