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After microinjection hemimethylated DNA is converted into symmetrically methylated DNA before DNA replication.
Sandberg, G; Guhl, E; Graessmann, M; Graessmann, A.
Afiliação
  • Sandberg G; Institut für Molekularbiologie und Biochemie, Freien Universitt Berlin, Germany.
FEBS Lett ; 283(2): 247-50, 1991 Jun 03.
Article em En | MEDLINE | ID: mdl-1646121
In this investigation we analysed the maintenance methylation activity of the mammalian cell DNA methyltransferase by microinjection of hemimethylated HSV-tk DNA into thymidine kinase-negative rat 2 cells. We found that the hemimethylated DNA was efficiently converted into symmetrical methylated molecules before DNA replication. Furthermore, integration of the trans-DNA into the host genome is an early event after gene transfer.
Assuntos
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Base de dados: MEDLINE Assunto principal: DNA de Cadeia Simples / DNA Viral / Simplexvirus / Replicação do DNA Limite: Animals Idioma: En Ano de publicação: 1991 Tipo de documento: Article
Buscar no Google
Base de dados: MEDLINE Assunto principal: DNA de Cadeia Simples / DNA Viral / Simplexvirus / Replicação do DNA Limite: Animals Idioma: En Ano de publicação: 1991 Tipo de documento: Article