Mesencephalic astrocyte-derived neurotrophic factor enhances nigral gamma-aminobutyric acid release.
Neuroreport
; 17(3): 293-7, 2006 Feb 27.
Article
em En
| MEDLINE
| ID: mdl-16462600
ABSTRACT
Mesencephalic astrocyte-derived neurotrophic factor (MANF) - one of a new class of astrocyte-derived human proteins--selectively promotes the survival of dopamine neurons of the ventral midbrain. Using the whole-cell clamp technique, we looked for acute effects of MANF on gamma-aminobutyric acid type A (GABAA) receptor-mediated inhibitory postsynaptic currents (IPSCs) in dopamine neurons of the substantia nigra pars compacta of 6 to 15-day-old rats. In slices, MANF increased the amplitude of evoked IPSCs and decreased the paired pulse ratio. In mechanically dissociated cells, MANF increased the frequency of spontaneous and miniature IPSCs, without changing their mean amplitudes; and in enzymatically dissociated neurons, MANF had no effect on currents induced by exogenous GABA. The presynaptic enhancement of GABAergic inhibition may contribute to MANF's protective action on dopamine cells.
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Base de dados:
MEDLINE
Assunto principal:
Mesencéfalo
/
Fator Neurotrófico Derivado de Linhagem de Célula Glial
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Ácido gama-Aminobutírico
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Inibição Neural
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Neurônios
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article