Developmental expression of Manduca shade, the P450 mediating the final step in molting hormone synthesis.
Mol Cell Endocrinol
; 247(1-2): 166-74, 2006 Mar 09.
Article
em En
| MEDLINE
| ID: mdl-16473459
ABSTRACT
The ecdysone 20-monooxygenase (E20MO; 20-hydroxylase) is the enzyme that mediates the conversion of ecdysone (E) to the active insect molting hormone, 20-hydroxyecdysone (20E), which coordinates developmental progression. We report the identification and developmental expression of the Halloween gene shade (shd; CYP314A1) that encodes the E20MO in the tobacco hornworm, Manduca sexta. Manduca Shd (MsShd) mediates the conversion of E to 20E when expressed in Drosophila S2 cells. In accord with the central dogma, the data show that Msshd is expressed mainly in the midgut, Malpighian tubules, fat body and epidermis with very low expression in the prothoracic gland and nervous system. Developmental variations in E20MO enzymatic activity are almost perfectly correlated with comparable changes in the gene expression of Msshd in the fat body and midgut during the fifth instar and the beginning of pupal-adult development. The results indicate three successive and overlapping peaks of expression in the fat body, midgut and Malpighian tubules, respectively, during the fifth larval instar. The data suggest that precise tissue-specific transcriptional regulation controls the levels, and thereby the activity, of the Manduca E20MO.
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Base de dados:
MEDLINE
Assunto principal:
Esteroide Hidroxilases
/
Hidrocarboneto de Aril Hidroxilases
/
Manduca
Limite:
Animals
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article