The IGF-I/IGFBP-3 system in gingival crevicular fluid and dependence on application of fixed force.
J Endocrinol Invest
; 28(11): 1009-14, 2005 Dec.
Article
em En
| MEDLINE
| ID: mdl-16483180
BACKGROUND: During application of orthodontic force on the tooth, various molecular parameters associated with tissue remodeling are changed. IGF-I is a regulatory protein produced during periodontal regeneration. IGF binding proteins-3 (IGFBP-3), a specific IGF-I binding protein, is the major regulatory factor of IGF-I activity. OBJECTIVES: We tested the hypothesis that changes in the IGF-I/ IGFBP-3 system occur during fixed force application to the tooth and that these changes are detectable in the gingival crevicular fluid (GCF). METHODS: IGFBP-3 and IGF-I secretion into gingival crevicular fluid (GCF) was analyzed by Western blotting and immunoradiometric assay (IRMA), respectively, in GCF of 6 healthy subjects just prior to and during orthodontics treatment using fixed appliances. RESULTS: We observed a significant time-dependent decrease of IGFBP-3 content in GCF during orthodontic treatment (4 h and 10 days). Reduction in levels of intact, glycosylated 47 kDa form of IGFBP-3 was associated with its degradation and the appearance of intermediate breakdown products. IGF-I levels were significantly increased 4 h after application of orthodontic force, while they were significantly reduced 10 days after the start of treatment. CONCLUSIONS: IGFBP-3 secretion into GCF and its molecular structure are modified by the fixed force of orthodontic treatment. Alterations in IGFBP-3 appear to be unrelated to the binding to IGF-I, suggesting an IGF-independent role of this binding protein in tooth movement.
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Base de dados:
MEDLINE
Assunto principal:
Técnicas de Movimentação Dentária
/
Fator de Crescimento Insulin-Like I
/
Líquido do Sulco Gengival
/
Remodelação Óssea
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Proteína 3 de Ligação a Fator de Crescimento Semelhante à Insulina
Limite:
Adolescent
/
Adult
/
Child
/
Female
/
Humans
/
Male
Idioma:
En
Ano de publicação:
2005
Tipo de documento:
Article