Compact soft x-ray transmission microscopy with sub-50 nm spatial resolution.
Phys Med Biol
; 51(6): N99-107, 2006 Mar 21.
Article
em En
| MEDLINE
| ID: mdl-16510949
ABSTRACT
In this paper, the development of compact transmission soft x-ray microscopy (XM) with sub-50 nm spatial resolution for biomedical applications is described. The compact transmission soft x-ray microscope operates at lambda = 2.88 nm (430 eV) and is based on a tabletop regenerative x-ray source in combination with a tandem ellipsoidal condenser mirror for sample illumination, an objective micro zone plate and a thinned back-illuminated charge coupled device to record an x-ray image. The new, compact x-ray microscope system requires the fabrication of proper x-ray optical devices in order to obtain high-quality images. For an application-oriented microscope, the alignment procedure is fully automated via computer control through a graphic user interface. In imaging studies using our compact XM system, a gold mesh image was obtained with 45 nm resolution at x580 magnification and 1 min exposure. Images of a biological sample (Coscinodiscus oculoides) were recorded.
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Base de dados:
MEDLINE
Assunto principal:
Raios X
/
Microanálise por Sonda Eletrônica
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article