Fluorescence lifetime images and correlation spectra obtained by multidimensional time-correlated single photon counting.
Microsc Res Tech
; 69(3): 186-95, 2006 Mar.
Article
em En
| MEDLINE
| ID: mdl-16538624
ABSTRACT
Multidimensional time-correlated single photon counting (TCSPC) is based on the excitation of the sample by a high-repetition rate laser and the detection of single photons of the fluorescence signal in several detection channels. Each photon is characterized by its arrival time in the laser period, its detection channel number, and several additional variables such as the coordinates of an image area, or the time from the start of the experiment. Combined with a confocal or two-photon laser scanning microscope and a pulsed laser, multidimensional TCSPC makes a fluorescence lifetime technique with multiwavelength capability, near-ideal counting efficiency, and the capability to resolve multiexponential decay functions. We show that the same technique and the same hardware can be used for precision fluorescence decay analysis and fluorescence correlation spectroscopy (FCS) in selected spots of a sample.
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Base de dados:
MEDLINE
Assunto principal:
Microscopia de Fluorescência por Excitação Multifotônica
Limite:
Humans
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article