Distribution of calcineurin activity in blood cell fractions and impact of tacrolimus inhibition.
Fundam Clin Pharmacol
; 20(2): 137-44, 2006 Apr.
Article
em En
| MEDLINE
| ID: mdl-16573714
ABSTRACT
The aim of this study was to investigate calcineurin (PP2B) activity in different blood cell fractions and its inhibition by tacrolimus. Basal PP2B activity was measured in each blood cellular fraction collected from healthy volunteers. The inhibition profile of PP2B activity was explored in isolated peripheral blood mononuclear cells (PBMC) and platelets exposed directly to tacrolimus and in PBMC and platelets isolated from whole blood previously exposed to tacrolimus. Contrasting with red blood cells (30%) and platelets (25%), PBMC represented only 8.7% of PP2B activity of unfractionated whole blood. After tacrolimus exposure of isolated PBMC and platelets, the concentration of tacrolimus required to inhibit 50% of PP2B activity (EC(50)) in PBMC was significantly lower than in platelets (0.26 ng/mL vs. 0.83 ng/mL, P < 0.001). EC(50) values were similar in PBMC and platelets isolated from whole blood previously exposed to tacrolimus (7.69 ng/mL vs. 7.42 ng/mL, respectively). These results suggest PBMC is a very suitable matrix for PP2B measurement in monitoring transplant recipients but clinical studies are necessary to solve clearly this issue.
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Base de dados:
MEDLINE
Assunto principal:
Plaquetas
/
Tacrolimo
/
Calcineurina
/
Eritrócitos
/
Imunossupressores
/
Leucócitos
Limite:
Humans
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article