Comparison of standard surface chemistries for culturing mesenchymal stem cells prior to neural differentiation.

ABSTRACT

A critical element of any stem cell differentiation protocol is the ability to compare its effects relative to an undifferentiated population of the same cells. In an attempt to standardize pre-differentiation conditions of adult derived mesenchymal stem cells prior to neural induction experiments, we asked what is the simplest chemical surface that supports the growth and maintenance of these cells in a pre-differentiation state. Adult bone marrow-derived rat mesenchymal stem cells (BMSCs) were expanded in vitro on Permanox Lab-Tek tissue culture treated plastic (TCP), poly-D-lysine (PDL) coated glass, PDL-laminin-1 coated glass, and untreated glass. TCP provided the best surface for maintaining morphologies generally considered to be undifferentiated, while PDL coated glass and uncoated glass provided the least suitable surfaces. Expansion of BMSCs on PDL-laminin-1 coated glass resulted in expression of nestin, a marker associated with neuronal and other progenitor cells, and therefore may confound experimental results if used as a pre-differentiation surface.