Identification of HLA-A*0201-presented T cell epitopes derived from the oncofetal antigen-immature laminin receptor protein in patients with hematological malignancies.
J Immunol
; 176(11): 6935-44, 2006 Jun 01.
Article
em En
| MEDLINE
| ID: mdl-16709854
ABSTRACT
The oncofetal Ag immature laminin receptor (OFA-iLR) is a potential target molecule for immunotherapeutic studies in several tumor entities, including hematological malignancies. In the present study, we characterize two HLA-A*0201-presented epitopes eliciting strong OFA-iLR peptide-specific human cytotoxic T cell (CTLs) responses in vitro. Both allogeneic HLA-A*0201-matched and autologous CTLs recognized and killed endogenously OFA-iLR-expressing tumor cell lines and primary malignant cells from patients with hemopoietic malignancies in an MHC-restricted fashion but spared nonmalignant hemopoietic cells. Spontaneous OFA-iLR peptide-specific T cell reactivity was detectable in a significant proportion of leukemia patients. Interestingly, in patients with chronic lymphocytic leukemia and multiple myeloma but not in those with acute myeloid leukemia, significant frequencies of OFA peptide-specific CTLs could be detected in an early stage of disease but disappeared in patients with progressive disease. The identification of OFA-iLR-derived peptide epitopes provides a basis for tumor immunological studies and therapeutic vaccination strategies in patients with OFA-iLR-expressing malignancies.
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Base de dados:
MEDLINE
Assunto principal:
Antígenos HLA-A
/
Leucemia Linfocítica Crônica de Células B
/
Leucemia Mieloide Aguda
/
Receptores de Laminina
/
Apresentação de Antígeno
/
Epitopos de Linfócito T
/
Mieloma Múltiplo
/
Antígenos de Neoplasias
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article