Apomyoglobin reveals a random-nucleation mechanism in amyloid protofibril formation.
Acta Histochem
; 108(3): 215-9, 2006.
Article
em En
| MEDLINE
| ID: mdl-16714052
ABSTRACT
Protofibrils (PFs) represent the earliest fibrillar species that occur in the course of amyloid fibril formation. Using apomyoglobin, we report here that PFs arise from a multi-step reaction and that they are preceded by an ensemble of non-fibrillar particles (NFPs). These intermediate aggregates encompass nascent elements of amyloid structure and can act as seeds in PF formation. Taken together with the observation that PFs often protrude from NFPs, our data suggest that PFs form by a random nucleation mechanism in which the polypeptide chains sample many different aggregated conformations. Once the appropriate structural characteristics are acquired, PFs are formed by addition of further polypeptide chains.
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Base de dados:
MEDLINE
Assunto principal:
Apoproteínas
/
Amiloide
/
Mioglobina
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article