Spatial distribution of nerve sprouting after myocardial infarction in mice.
Heart Rhythm
; 3(6): 728-36, 2006 Jun.
Article
em En
| MEDLINE
| ID: mdl-16731479
ABSTRACT
BACKGROUND:
Myocardial infarction (MI) elicits nerve sprouting.OBJECTIVES:
The purpose of this study was to determine the spatial distribution of nerve sprouting and neurotrophic gene expression after MI.METHODS:
We created MI in mice by coronary artery ligation. The hearts were removed 3 hours to 2 months after MI and examined for nerve fiber density and neurotrophic factor gene expression using Affymetrix microarray and mRNA analyses.RESULTS:
The density of nerve fibers immunopositive for growth-associated protein (GAP)-43 was the highest 3 hours after MI both in the peri-infarct area and in the area remote to infarct, resulting in sympathetic (but not parasympathetic) hyperinnervation in the ventricles. The GAP-43-positive nerve fiber density of myocardium was greater in the outer transverse loop than in the inner vertical loop. The differences between these two myocardial loops peaked within 3 hours after MI and persisted for 2 months afterward. Gene expression of nerve growth factor, insulin-like growth factor, leukemia inhibitory factor, transforming growth factor-beta(3), and interleukin-1alpha was increased up to 2 months after MI compared with normal control. Expression of these growth factors was more pronounced and persistent in the peri-infarct area than in the remote area.CONCLUSION:
MI induces sympathetic nerve sprouting in both peri-infarct and remote areas, more in the outer transverse loop. Selective up-regulation of nerve growth factor, insulin-like growth factor, leukemia inhibitory factor, transforming growth factor-beta(3), and interleukin-1alpha occurred in the peri-infarct area and, to a lesser extent, in the remote area.
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Base de dados:
MEDLINE
Assunto principal:
Sistema Nervoso Simpático
/
Coração
/
Infarto do Miocárdio
/
Regeneração Nervosa
Limite:
Animals
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article