The Drosophila NuMA Homolog Mud regulates spindle orientation in asymmetric cell division.
Dev Cell
; 10(6): 731-42, 2006 Jun.
Article
em En
| MEDLINE
| ID: mdl-16740476
ABSTRACT
During asymmetric cell division, the mitotic spindle must be properly oriented to ensure the asymmetric segregation of cell fate determinants into only one of the two daughter cells. In Drosophila neuroblasts, spindle orientation requires heterotrimeric G proteins and the G alpha binding partner Pins, but how the Pins-G alphai complex interacts with the mitotic spindle is unclear. Here, we show that Pins binds directly to the microtubule binding protein Mud, the Drosophila homolog of NuMA. Like NuMA, Mud can bind to microtubules and enhance microtubule polymerization. In the absence of Mud, mitotic spindles in Drosophila neuroblasts fail to align with the polarity axis. This can lead to symmetric segregation of the cell fate determinants Brat and Prospero, resulting in the mis-specification of daughter cell fates and tumor-like over proliferation in the Drosophila nervous system. Our data suggest a model in which asymmetrically localized Pins-G alphai complexes regulate spindle orientation by directly binding to Mud.
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Base de dados:
MEDLINE
Assunto principal:
Polaridade Celular
/
Proteínas de Insetos
/
Proteínas de Drosophila
/
Antígenos Nucleares
/
Proteínas Associadas à Matriz Nuclear
/
Drosophila
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Proteínas de Membrana
/
Fuso Acromático
/
Proteínas do Tecido Nervoso
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article