Usefulness of the SOS Chromotest in the study of medicinal plants as radioprotectors.
Int J Radiat Biol
; 82(5): 323-9, 2006 May.
Article
em En
| MEDLINE
| ID: mdl-16782649
ABSTRACT
PURPOSE:
The aim of this work is to investigate the usefulness of a modified protocol of the SOS Chromotest to detect antigenotoxicity activities against gamma-rays of plant extracts with proven antioxidant activity, and to elucidate the antigenotoxic mechanisms involved in radioprotection using this system. MATERIALS ANDMETHODS:
The methodology developed was assayed with amifostine, the most studied radioprotector, and with Phyllanthus orbicularis HBK, Cymbopogon citratus (DC) Stapf and Pinus caribaea Morelet extracts, using pre- and post-treatment procedures.RESULTS:
The P. caribaea and C. citratus extracts were antigenotoxic against gamma-rays when the cells were pre-treated with both extracts, suggesting a possible antigenotoxic action through a free radical scavenging mechanisms. Amifostine and the P. orbicularis extract were also antigenotoxic under pre- and post-treatment conditions, indicating that several antimutagenic components of this plant extract may also operate by some intracellular mechanism, unlike its antioxidant activity.CONCLUSIONS:
The results have demonstrated the usefulness of the modified SOS Chromotest assay in the screening of phytochemical radioprotectors as well as in the study of their antimutagenic mechanisms.
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Base de dados:
MEDLINE
Assunto principal:
Plantas Medicinais
/
Resposta SOS em Genética
/
Extratos Vegetais
/
Escherichia coli
/
Testes de Mutagenicidade
Tipo de estudo:
Guideline
Idioma:
En
Ano de publicação:
2006
Tipo de documento:
Article