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The generation of thymus-independent germinal centers depends on CD40 but not on CD154, the T cell-derived CD40-ligand.
Gaspal, Fabrina M C; McConnell, Fiona M; Kim, Mi-Yeon; Gray, David; Kosco-Vilbois, Marie H; Raykundalia, Chandra R; Botto, Marina; Lane, Peter J L.
Afiliação
  • Gaspal FM; MRC Centre for Immune Regulation, University of Birmingham, Birmingham, UK.
Eur J Immunol ; 36(7): 1665-73, 2006 Jul.
Article em En | MEDLINE | ID: mdl-16783845
In this report, we show that the formation of germinal center (GC)-like structures to thymus-independent type 2 antigens in mice depends on intact signals through CD40, but does not depend on T cell-derived CD40-ligand (CD154). In addition, we show that follicular dendritic cells (FDC) are also critical to thymus-independent GC formation, as their depletion by blockade of lymphotoxin-beta receptor signals abrogated GC development unless the responding B cells bound antigen with high affinity. Further evidence that immune complexes drove this CD40-dependent B cell proliferation was provided by the observation that an antibody that detects immune complexes containing complement component 4 on FDC also inhibited thymus-independent GC formation when injected in vivo at the time of immunization. Finally, we show that thymus-independent B cell proliferation was associated with class switching to IgG3, as IgG3(+) antigen-specific switched B cells could be visualized directly in GC, suggesting that immune complexes can provide the signals for class switching within GC in the absence of CD154.
Assuntos
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Base de dados: MEDLINE Assunto principal: Timo / Centro Germinativo / Antígenos CD40 / Ligante de CD40 / Antígenos T-Independentes Limite: Animals Idioma: En Ano de publicação: 2006 Tipo de documento: Article
Buscar no Google
Base de dados: MEDLINE Assunto principal: Timo / Centro Germinativo / Antígenos CD40 / Ligante de CD40 / Antígenos T-Independentes Limite: Animals Idioma: En Ano de publicação: 2006 Tipo de documento: Article